Journal: Bio-protocol
Article Title: An Ex Vivo Lung Histoculture Model for Studying Pulmonary Infection and Immune Response With SARS-CoV-2 as an Example of RNA Virus
doi: 10.21769/BioProtoc.5552
Figure Lengend Snippet: Representative step-by-step processing of an autopsy lung plated into histoculture. (A) Gelfoam ® gelatin sponge is cut and first placed in complete cell medium (dry sponges). (B) Sponge is soaked in complete cell medium using a sterile spatula. (C) Properly soaked sponge completely immersed in the medium. (D) Representative lung explant examined by a pathologist intended for histoculture, sterile tweezers, and scalpel with a sharp disposable blade. (E) Explant size. (F) The 2 × 2 × 2 mm tissue blocks should be cut in a sufficient amount for all experimental procedures and then mixed. (G) Pre-wet sponges placed in the center of each culture well with 2 mL of complete cell medium. (H) Explants placed on a pre-wet sponge. (I) Addition of the final 1 mL of complete cell medium to place the explants at the air–liquid interface.
Article Snippet: Gelfoam ® hemostatic agent absorbable gelatin topical sponge (Pfizer, catalog number: 00009031508); store at RT 3.
Techniques: Sterility